A method and apparatus for the sanitization, detoxification, disinfection, high level disinfection, or sterilization of both the interior and exterior surfaces of an object or plurality of objects, including those with lumens and/or channels of various sizes, within a closed space, or closed system of space, in addition to their surrounding atmosphere, and relates particularly, though not exclusively, to the generation of an aerosol including an anti-pathogen/toxin/fungal/sporicidal agent(s) or substance(s) (applied agent), or chemical neutralizing agent(s) or substance(s), by way of one or more ultrasonic nebulizer(s). However the applied agent used in the present invention may also be in the form of any gas, vapor, plasma, aerosol, or other form. The said apparatus and method also includes an option to incorporate and utilize a means to wash the object(s) or endoscope(s).