An object is to provide: a method for determining a tongue cancer in which the malignancy of tongue cancer can be objectively and accurately determined; a method for analyzing a tongue cancer tissue specimen; and a kit for analyzing a tongue cancer tissue specimen. There is provided: a method for determining a tongue cancer, comprising measuring mRNA quantity of an integrin family gene and a reference gene in the tongue cancer tissue specimen, and determining the malignancy of the tongue cancer based on a ratio of the mRNA quantity of the integrin family gene/the mRNA quantity of the reference gene; a method for analyzing a tongue cancer tissue specimen, comprising the steps of measuring mRNA quantity of an integrin family gene and a reference gene in the tongue cancer tissue specimen, and correlating a ratio of the mRNA quantity of the integrin family gene/the mRNA quantity of the reference gene with clinical data; and a kit for analyzing a tongue cancer tissue specimen.