A label-free interferometric biosensor is disclosed which is based on the self-mixing optical interferometer. Inside the biosensor, an incoming beam is divided into two beam portions which pass through a channel and bio materials, respectively. Interference of the portions is realized by the self-mixing effect and used to detect existence of an analyte, such as DNA or protein molecules. The label-free biosensor is compact and can be made on a chip using the semiconductor technology. It is also convenient to use due to moderate alignment requirement. Furthermore, an array of the interferometers fabricated on a chip enables high-throughput and highly parallel measurements.