Various embodiments of a device for applying a substance to eyelashes and/or eyebrows may include a receptacle containing the substance and an applicator including an applicator member. In some examples, the applicator member may include a twisted support extending along a longitudinal axis and bristles held by the support. The applicator member may define at least one first longitudinally-extending region having a succession of notches configured to be loaded with the substance and at least one second longitudinally-extending region configured to separate eyelashes and/or eyebrows. In some exemplary embodiments, the first region may include at least five notches.