An opto-electric hybrid board in which a new alignment mark having an identifying mark that is easy to recognize is formed in addition to a conventional alignment mark, and a method of manufacturing the opto-electric hybrid board. The opto-electric hybrid board includes an optical waveguide portion 2, an electric circuit board 1, and optical elements mounted on this electric circuit board 1. The optical waveguide portion 2 includes a translucent under cladding layer 21, a linear core 22 for an optical path, first alignment marks 24 positioned relative to end portions of this core 22, and an over cladding layer 23 for covering the above-mentioned core 22 and the first alignment marks 24. The electric circuit board 1 includes second alignment marks 15 for positioning of the optical elements and formed on a surface thereof on which the optical elements are mounted.