Invention Grant
- Patent Title: Methods of cleaving DNA with rationally-designed meganucleases
- Patent Title (中): 用合理设计的大核苷酸切割DNA的方法
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Application No.: US13246376Application Date: 2011-09-27
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Publication No.: US08148098B2Publication Date: 2012-04-03
- Inventor: James Jefferson Smith , Derek Jantz , Homme W. Hellinga
- Applicant: James Jefferson Smith , Derek Jantz , Homme W. Hellinga
- Applicant Address: US NC Durham
- Assignee: Duke University
- Current Assignee: Duke University
- Current Assignee Address: US NC Durham
- Agency: Wilmer Cutler Pickering Hale and Dorr LLP
- Main IPC: C12Q1/34
- IPC: C12Q1/34 ; C12Q1/68 ; C07K14/00 ; C12N9/16 ; C12P21/00
Abstract:
Rationally-designed LAGLIDADG meganucleases and methods of making such meganucleases are provided. In addition, methods are provided for using the meganucleases to generate recombinant cells and organisms having a desired DNA sequence inserted into a limited number of loci within the genome, as well as methods of gene therapy, for treatment of pathogenic infections, and for in vitro applications in diagnostics and research.
Public/Granted literature
- US20120040406A1 RATIONALLY-DESIGNED MEGANUCLEASES WITH ALTERED SEQUENCE SPECIFICITY AND DNA-BINDING AFFINITY Public/Granted day:2012-02-16
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