An amphibious aircraft trailer includes a frame supported on a wheel/suspension bearing axle for movements over a roadway surface. Incorporated into the trailer is a cradle configured to engage a hull of the amphibious aircraft when it is loaded and unloaded in water conditions. A lift mechanism interposed between and coupled to the frame and the cradle is operable to displace the cradle vertically with respect to the frame such that landing gear associated with the amphibious aircraft can be extended or retracted while on the trailer. The trailer may also include a plurality of wheel tracks aligned with the landing gear to facilitate the aircraft's loading and unloading in its ground based mode of operation.