Invention Grant
US08153372B2 Method for simultaneously determining in a single multiplex reaction gender of donors and quantities of genomic DNA and ratios thereof, presence and extent of DNA degradation, and PCR inhibition within a human DNA sample
有权
在单次多重反应中同时确定供体的性别和基因组DNA的量及其比例，DNA降解的存在和程度以及人类DNA样品内的PCR抑制的方法
- Patent Title: Method for simultaneously determining in a single multiplex reaction gender of donors and quantities of genomic DNA and ratios thereof, presence and extent of DNA degradation, and PCR inhibition within a human DNA sample
- Patent Title (中): 在单次多重反应中同时确定供体的性别和基因组DNA的量及其比例，DNA降解的存在和程度以及人类DNA样品内的PCR抑制的方法
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Application No.: US11960113Application Date: 2007-12-19
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Publication No.: US08153372B2Publication Date: 2012-04-10
- Inventor: Robert W. Allen , Valerie Fuller
- Applicant: Robert W. Allen , Valerie Fuller
- Applicant Address: US OK Stillwater
- Assignee: The Board of Regents for Oklahoma State University
- Current Assignee: The Board of Regents for Oklahoma State University
- Current Assignee Address: US OK Stillwater
- Agency: Fellers, Snider, Blankenship, Bailey & Tippens, P.C.
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12P19/34
Abstract:
Methods are provided for determining, in a single polymerase chain reaction (PCR) reaction, the quantity, quality, and gender of origin of DNA in a sample, and whether or not the sample contains PCR amplification inhibitors. The methods involve carrying out a single PCR multiplex reaction utilizing primer sets specific for amplifying the human amelogenin locus, an X- and/or Y-chromosome specific gene that is shorter than the amelogenin gene, and a heterologous, non-human reporter gene.
Public/Granted literature
- US20080153099A1 Quantitation of Human Genomic DNA Public/Granted day:2008-06-26
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