Invention Grant
- Patent Title: Method of making activated carboxypeptidases
- Patent Title (中): 活化羧肽酶的制备方法
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Application No.: US12312663Application Date: 2007-11-21
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Publication No.: US08153395B2Publication Date: 2012-04-10
- Inventor: Per Nørgaard
- Applicant: Per Nørgaard
- Applicant Address: DK Bagsvaerd
- Assignee: Novo Nordisk A/S
- Current Assignee: Novo Nordisk A/S
- Current Assignee Address: DK Bagsvaerd
- Agent Michael J. Brignati
- Priority: EP06124576 20061122
- International Application: PCT/EP2007/062629 WO 20071121
- International Announcement: WO2008/062010 WO 20080529
- Main IPC: C12P21/06
- IPC: C12P21/06
Abstract:
The invention is related to a method for making an activated carboxypeptidase in a fungi cell comprising introducing a DNA sequence encoding a proform of the carboxypeptidase wherein a Kex2 site has been introduced in the prosequence of the carboxypeptidase, culturing the fungi cell under conditions suitable for expression of the procarboxypeptidase and cleaving off the prosequence within the cell to liberate the free active form of the carboxypeptidase. The invention is also related to methods for making mature human insulin and human insulin analogues by use of the activated carboxypeptidase enzyme.
Public/Granted literature
- US20100173357A1 METHOD OF MAKING ACTIVATED CARBOXYPEPTIDASES Public/Granted day:2010-07-08
Information query
