An observation device observing a sample cultured in a culture vessel includes an illuminating unit including an illumination optical system and illuminating the sample, an image-capturing unit including an imaging sensor and generating an image by capturing an image of the sample illuminated by the illuminating unit, and a wavelength limiting filter being placed on an optical axis of the illumination optical system and between the illuminating unit and the image-capturing unit, and limiting a part of wavelengths of an illumination light from the illumination optical system in accordance with optical absorption properties of an additive contained in a culture medium used for culturing the sample. Accordingly, it is possible to suppress a change of the image resulting from the additive and to enable to generate an appropriate image in an automatic observation.