An ultrasound training assembly includes a tubular member that has a first end, a second end and a perimeter wall extending between the first and second ends. The perimeter wall has an aperture therein. A model representing an ultrasound probe includes a grip and a head attached together. The head has a distal edge with respect to the grip. The distal edge is elongated. An elongated member is attached to the distal edge and has a longitudinal axis orientated perpendicular to a longitudinal axis of the model extending through the distal edge and a free end of the grip. The elongated member is spaced from the distal edge a distance between 0.5 inches and 2 inches. The elongated member is placed in the tubular member through the aperture to represent a vein which would be seen by an ultrasound probe represented by the model.