Invention Grant
- Patent Title: Method and apparatus for high speed carbohydrate analysis
- Patent Title (中): 高速碳水化合物分析方法和装置
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Application No.: US11983814Application Date: 2007-11-09
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Publication No.: US08163152B1Publication Date: 2012-04-24
- Inventor: Varouj Amirkhanian , Ming-Sun Liu , Andras Guttman
- Applicant: Varouj Amirkhanian , Ming-Sun Liu , Andras Guttman
- Applicant Address: US MD Germantown
- Assignee: Qiagen Sciences, LLC
- Current Assignee: Qiagen Sciences, LLC
- Current Assignee Address: US MD Germantown
- Agency: Liu & Liu
- Main IPC: G01N27/447
- IPC: G01N27/447 ; G01N27/453

Abstract:
A cost-effective multi-channel capillary gel-electrophoresis system for highly efficient, high speed, high throughput, carbohydrate analysis. In one aspect of the present invention, a high-performance capillary electrophoresis analyzer system has been optimized for carbohydrate analysis application. The system uses a multiplexed/time-staggered fluorescence type detection mechanism, with an integrated fiber optic array-based technology and a novel disposable gel-cartridge. Twelve carbohydrate samples are automatically injected, electrophoretically separated, detected and analyzed simultaneously by using a multiple usage and disposable multi-capillary gel-cartridge. Using commercially available labeling agent such as APTS (representing 8-Aminopyrene-1,3,6-trisulfonic acid, trisodium salt) as an indicator, the capillary electrophoresis-based carbohydrate system/analyzer provides high resolving power in 2-15 minutes of separations. The system can hold a total of 96 samples, which can be automatically analyzed within 30-60 minutes. This affordable fiber optic based fluorescence detection system can be used in laboratories for high speed carbohydrate sequencing & screening applications.
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