A perfume bottle sealing structure includes a cover, which is covered on the bottleneck of a perfume bottle and has an air hole and a tubular center shaft defining a longitudinal fluid passage, and a seal that is fastened to the top end of the tubular center shaft to seal the longitudinal fluid passage and the air hole during transportation of the perfume bottle and, which has a plug rod and a bottom pin respectively fitted into the longitudinal fluid passage and the air hole to seal the bottleneck when the seal is fastened to the cover.