It is an object of this invention to provide a sample integration cassette, a spotting device and a sample integration device which enhance a sample integration density and reduce the size of a sample assembly and a probe. The sample integration cassette has the sample assembly and the sample support carrier so arranged therein that the sample support can be fed from the sample support carrier onto the sample assembly at a predetermined angle to the sample assembly at all times. The cassette is formed so as to be connectable with a drive unit that applies a drive force to the sample assembly and the sample support carrier interlockingly and synchronously. The spotting device has a delivery member mounting table which is vertically moved over the sample support carrier to apply samples to the sample support from the delivery member. The sample integration device rotatably supports the sample support carrier and includes a rotary drive unit for rotating the sample support carrier and the sample assembly and a biasing means connected to the rotary drive unit to pull the cassette in one direction. The sample assembly rotating device includes a rotating shaft to which the sample assembly is fitted at one end, a drive means to drive the rotating shaft, and a power supply for the drive means.