Invention Grant
- Patent Title: Preparation of nucleic acid templates for solid phase amplification
- Patent Title (中): 制备用于固相扩增的核酸模板
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Application No.: US12085508Application Date: 2006-11-24
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Publication No.: US08168388B2Publication Date: 2012-05-01
- Inventor: Niall Anthony Gormley , Jonathan Mark Boutell , Gerardo Turcatti , Colin Lloyd Barnes
- Applicant: Niall Anthony Gormley , Jonathan Mark Boutell , Gerardo Turcatti , Colin Lloyd Barnes
- Applicant Address: GB Essex
- Assignee: Illumina Cambridge Ltd
- Current Assignee: Illumina Cambridge Ltd
- Current Assignee Address: GB Essex
- Agent Tiffany B. Thomas
- Priority: GB0524069.2 20051125
- International Application: PCT/GB2006/004407 WO 20061124
- International Announcement: WO2007/060456 WO 20070531
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12P19/34

Abstract:
The invention relates to a method of preparing template constructs for solid-phase nucleic acid amplification and to use of the templates in methods of solid-phase nucleic acid amplification. The method involves carrying out two ligation reactions: (a) a ligation reaction in which the first end of one or more target polynucleotide molecules are ligated to surface-bound adaptor polynucleotide molecules, and (b) a ligation reaction in which solution-phase adaptor polynucleotide molecules are ligated to the second end of said target polynucleotide molecules, in order to produce one or more template constructs attached to a solid support.
Public/Granted literature
- US20100041561A1 Preparation of Nucleic Acid Templates for Solid Phase Amplification Public/Granted day:2010-02-18
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