A display substrate includes a first pixel part including a first switching element, a second pixel part including a second switching element, a third pixel part including a third switching element, a first pixel electrode, a second pixel electrode and a third pixel electrode. The third pixel part is adjacent to the first pixel part. The first pixel electrode is electrically connected to the first switching element, and is formed on the first and second pixel part. The second pixel electrode is electrically connected to the second switching element, and is formed on a portion of the second pixel part. The third pixel electrode is electrically connected to the third switching element, and is formed on the first and third pixel parts. Therefore, an opening ratio is increased, thereby improving an image display quality.