An anti-fatigue flooring system includes at least one anti-fatigue area adjacent a work station in a room. The anti-fatigue area includes an anti-fatigue member of a resilient foam material and transition members located about the anti-fatigue member. Flooring extends over the anti-fatigue member and the transition members so that the anti-fatigue member and the transition members are located between the flooring and the subfloor. The transition members transition the flooring from the top of the anti-fatigue member down to the subfloor located about the transition members. The flooring is preferably secured to the top of the anti-fatigue member, the top of the transition members, and the subfloor about the anti-fatigue area.