A toothbrush having needle-shaped bristles with various end points and a method of manufacturing the same. The needle-shaped toothbrush bristle, folded in an asymmetric U-shape to outwardly expose both ends thereof at a height difference ranging from 0.5 to 2 mm and disposed on a head portion of the toothbrush, has a tapered length of 3-7 mm and a blunt end point with a diameter of 0.01-0.07 mm. The toothbrush is advantageous in terms of high insertion function into the periodontal pockets of the user while leaving the user with a refreshed feeling after brushing his/her teeth.