A drill guide assembly in accordance with the present invention provide a bore in a tire. The drill guide assembly includes a first slide rail for lying on the tire when the tire lies on a side and a second slide rail for lying on the tire when the tire lies on a side. The second slide rail is disposed parallel to the first slide rail. A first engagement block is slidingly secured to the first slide rail and the second slide rail. The first engagement block engages a first shoulder portion of a tread portion of the tire. A second engagement block is slidingly secured to the first slide rail and the second slide rail. The second engagement block engages a second shoulder portion of the tread portion of the tire. The second shoulder portion is diametrically opposed to the first shoulder portion. A drill mount positions a drill relative to the tire. The drill mount includes a toroidal base, a first upright guide member secured to the toroidal base, a second upright guide member secured to the toroidal base, and a slide member for slidingly engaging the first and second guide members. The slide member has two outer cylindrical tubes for sliding along the first and second guide members and a central cylindrical tube for slidingly engaging a drill.