Invention Grant
- Patent Title: Granular bioplastic biocontrol composition
- Patent Title (中): 颗粒状生物塑料生物防治组合物
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Application No.: US12703257Application Date: 2010-02-10
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Publication No.: US08173179B1Publication Date: 2012-05-08
- Inventor: Hamed K. Abbas , Cesare Accinelli , Robert M. Zablotowicz , Maria L. Sacca , Jeff Wilkinson
- Applicant: Hamed K. Abbas , Cesare Accinelli , Robert M. Zablotowicz , Maria L. Sacca , Jeff Wilkinson
- Applicant Address: US DC Washington
- Assignee: The United States of America, as represented by the Secretary of Agriculture
- Current Assignee: The United States of America, as represented by the Secretary of Agriculture
- Current Assignee Address: US DC Washington
- Agent John D. Fado; Evelyn M. Rabin
- Main IPC: A01N65/00
- IPC: A01N65/00

Abstract:
Previous research demonstrated that aflatoxin contamination in corn is reduced by field application of wheat grains pre-inoculated with the non-aflatoxigenic Aspergillus flavus strain NRRL 30797. To facilitate field applications of the biocontrol isolate, a series of laboratory studies were conducted on the reliability and efficiency of replacing wheat grains with the novel bioplastic formulation Mater-Bi® to serve as a carrier matrix to formulate this fungus. Mater-Bi® granules were inoculated with a conidial suspension of NRRL 30797 to achieve a final cell density of ˜log 7 conidia/granule. Incubation of 20-g soil samples receiving a single Mater-Bi® granule for 60-days resulted in log 4.2 to 5.3 propagules of A. flavus/g soil for microbiologically active and sterilized soil, respectively. Increasing the number of granules had no effect on the degree of soil colonization by the biocontrol fungus. In addition to the maintenance of rapid vegetative growth and colonization of soil samples, the bioplastic formulation was highly stable, indicating that Mater-Bi® is a suitable substitute for biocontrol applications of A. flavus NRRL 30797.
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