A multi-layer coating structure with anti-reflection, anti-static and anti-smudge functions includes a substrate and a coating module. The coating module is formed on a front surface of the substrate and composed of a plurality of indium tin oxide compound coating layers and a plurality of silicon dioxide compound coating layers that are alternately stacked upon each other. The coating module further includes a fluorocarbon compound coating layer that is the uppermost layer of the coating module. Because the surface layer has good electrical conductive properties, the surface layer reduces much of the work in the grounding process and also increases the total yield and reliability in high volume production. The present invention provides a surface conductive layer structure with an anti-reflection coating that can be applied to the LCD and PDP display industries for glass and plastic film substrates.