Invention Grant
- Patent Title: Method of assaying enzyme-mediated oxidative demethylation
- Patent Title (中): 测定酶介导的氧化去甲基化的方法
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Application No.: US12353723Application Date: 2009-01-14
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Publication No.: US08173386B2Publication Date: 2012-05-08
- Inventor: Russell Hart , Barbara Scheuer , Raymond Trievel
- Applicant: Russell Hart , Barbara Scheuer , Raymond Trievel
- Applicant Address: US MI Ann Arbor
- Assignee: Arbor Assays, LLC
- Current Assignee: Arbor Assays, LLC
- Current Assignee Address: US MI Ann Arbor
- Agency: Gifford, Krass, Sprinkle, Anderson & Citkowski, P.C.
- Main IPC: C12Q1/26
- IPC: C12Q1/26
Abstract:
Methods of assaying enzyme-mediated oxidative demethylation are provided according to embodiments of the present invention which includes combining, under reaction conditions, an oxidative demethylation enzyme, a substrate for the oxidative demethylation enzyme and a formaldehyde detection reagent. Detection of fluorescence is indicative of formaldehyde generated by oxidative demethylation of the substrate by the enzyme, the fluorescence resulting from reaction of formaldehyde and the formaldehyde detection reagent.
Public/Granted literature
- US20100184108A1 METHODS AND COMPOSITIONS RELATING TO ENZYME ASSAYS Public/Granted day:2010-07-22
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