Invention Grant
US08173415B2 Single cell microinjection using flexible and compliant fluidic channels and electroosmotic dosage control 有权
单细胞显微注射使用柔性和顺应性流体通道和电渗剂量控制

Single cell microinjection using flexible and compliant fluidic channels and electroosmotic dosage control
Abstract:
A microinjection device for injecting a target cell with a reagent. The microinjection device includes a flexible substrate; a target supply channel formed in the flexible substrate for receiving the target cell; a reagent supply channel formed in the flexible substrate for receiving the reagent; a suction capillary mounted within a suction channel formed in the flexible substrate, the suction capillary providing suction to the target supply channel for immobilizing the target cell within the target supply channel; an injection needle mounted within a needle channel formed in the flexible substrate, the injection needle being movable between injected and un-injected positions by deforming at least a part of the flexible substrate; and a plurality of electrodes embedded in the flexible substrate, the plurality of electrodes creating a voltage potential across the injection needle to move the reagent into the target when the injection needle is in the injected position.
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