Invention Grant
US08173415B2 Single cell microinjection using flexible and compliant fluidic channels and electroosmotic dosage control
有权
单细胞显微注射使用柔性和顺应性流体通道和电渗剂量控制
- Patent Title: Single cell microinjection using flexible and compliant fluidic channels and electroosmotic dosage control
- Patent Title (中): 单细胞显微注射使用柔性和顺应性流体通道和电渗剂量控制
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Application No.: US12576766Application Date: 2009-10-09
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Publication No.: US08173415B2Publication Date: 2012-05-08
- Inventor: Arash Noori , Ponnambalam R. Selvaganapathy
- Applicant: Arash Noori , Ponnambalam R. Selvaganapathy
- Applicant Address: CA Hamilton
- Assignee: McMaster University
- Current Assignee: McMaster University
- Current Assignee Address: CA Hamilton
- Agency: Bereskin & Parr LLP/S.E.N.C.R.L., s.r.l.
- Main IPC: C12M1/00
- IPC: C12M1/00 ; C12M3/00

Abstract:
A microinjection device for injecting a target cell with a reagent. The microinjection device includes a flexible substrate; a target supply channel formed in the flexible substrate for receiving the target cell; a reagent supply channel formed in the flexible substrate for receiving the reagent; a suction capillary mounted within a suction channel formed in the flexible substrate, the suction capillary providing suction to the target supply channel for immobilizing the target cell within the target supply channel; an injection needle mounted within a needle channel formed in the flexible substrate, the injection needle being movable between injected and un-injected positions by deforming at least a part of the flexible substrate; and a plurality of electrodes embedded in the flexible substrate, the plurality of electrodes creating a voltage potential across the injection needle to move the reagent into the target when the injection needle is in the injected position.
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