Invention Grant
- Patent Title: Method for evaluating oil-and-fat amount in seed and method for screening for plant exhibiting varied level of oil-and-fat content
- Patent Title (中): 种子油脂含量评估方法及筛选具有不同油脂含量的植物的方法
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Application No.: US12920098Application Date: 2009-02-23
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Publication No.: US08173435B2Publication Date: 2012-05-08
- Inventor: Chikara Ohto , Norihiro Mitsukawa , Makoto Hayashi
- Applicant: Chikara Ohto , Norihiro Mitsukawa , Makoto Hayashi
- Applicant Address: JP Aichi-ken
- Assignee: Toyota Jidosha Kabushiki Kaisha
- Current Assignee: Toyota Jidosha Kabushiki Kaisha
- Current Assignee Address: JP Aichi-ken
- Agency: Sughrue Mion, PLLC
- Priority: JP2008-048485 20080228
- International Application: PCT/JP2009/053782 WO 20090223
- International Announcement: WO2009/107820 WO 20090903
- Main IPC: G01N33/03
- IPC: G01N33/03 ; G01N33/92
Abstract:
This invention is intended to evaluate the oil-and-fat amount in a seed and genetic changes thereof. A fusion protein of an oil body-specific protein, such as the oleosin-GFP fusion protein, and a fluorescent protein is expressed, and the oil-and-fat amount in a plant seed and changes thereof are evaluated based on the fluorescent intensity in a cotyledon of a fluorescent protein, such as GFP.
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