Invention Grant
- Patent Title: Intracellular production of a nuclease
- Patent Title (中): 核酸酶的细胞内产生
-
Application No.: US13072980Application Date: 2011-03-28
-
Publication No.: US08178335B2Publication Date: 2012-05-15
- Inventor: Pei-Chung Hsieh
- Applicant: Pei-Chung Hsieh
- Applicant Address: US MA Ipswich
- Assignee: New England Biolabs, Inc.
- Current Assignee: New England Biolabs, Inc.
- Current Assignee Address: US MA Ipswich
- Agency: New England Biolabs, Inc
- Agent Harriet M. Strimpel
- Main IPC: C07H21/00
- IPC: C07H21/00 ; C12N15/00 ; C12N1/21 ; C12N5/10 ; C12P21/00 ; C12N9/16
Abstract:
Methods and compositions are provided that relate to obtaining a recombinant DNA and RNA cleaving nuclease. This involves the over-expression of a fusion protein between maltose-binding protein and a truncated nuclease in a soluble form in the cytoplasm of a host cell from which it can be readily extracted.
Public/Granted literature
- US20110189753A1 Intracellular Production of a Nuclease Public/Granted day:2011-08-04
Information query
