Invention Grant
- Patent Title: Nucleic acid isolation using polidocanol and derivatives
- Patent Title (中): 使用波多卡醇和衍生物进行核酸分离
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Application No.: US13314739Application Date: 2011-12-08
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Publication No.: US08192958B2Publication Date: 2012-06-05
- Inventor: Sigrid Adie , Hermann Leying , Nicole Nachbaur , Eberhard Russmann
- Applicant: Sigrid Adie , Hermann Leying , Nicole Nachbaur , Eberhard Russmann
- Applicant Address: US CA Pleasanton
- Assignee: Roche Molecular Systems, Inc.
- Current Assignee: Roche Molecular Systems, Inc.
- Current Assignee Address: US CA Pleasanton
- Agent Olga Kay
- Priority: EP06025539 20061211
- Main IPC: C12Q1/70
- IPC: C12Q1/70 ; C12Q1/68 ; C07C21/00
Abstract:
This invention relates to a composition comprising a chaotropic agent, a buffering substance, and 0.5 to 5% (V/V) polidocanol or a derivative thereof. The invention is further related to uses of this composition and to a kit comprising the composition according to the invention. The invention is further related to a method for the detection of a nucleic acid in a biological sample comprising the steps of incubating the biological sample in the presence of a chaotropic agent, a buffering substance, and 0.5 to 5% (V/V) polidocanol or a derivative thereof, optionally isolating the nucleic acid, optionally amplifying the nucleic acid, and detecting the nucleic acid. The invention is further related to a method for the purification of a nucleic acid in a biological sample comprising the steps of incubating the biological sample in the presence of a chaotropic agent, a buffering substance, and 0.5 to 5% (V/V) polidocanol or a derivative thereof and isolating the nucleic acid thereby purifying the nucleic acid.
Public/Granted literature
- US20120100599A1 Nucleic Acid Isolation Using Polidocanol and Derivatives Public/Granted day:2012-04-26
Information query
