An incubator including a temperature-controlled room adjusted to a predetermined environment condition for culturing a sample of an incubation container at inside of the temperature-controlled room, the incubator including a shifting mechanism for moving a position of the incubation container at inside of the temperature-controlled room by a motor, a temperature adjusting section for adjusting a temperature at inside of the temperature-controlled room, an operating information generating section for generating operating information with regard to a position of operating and a time period of operating the motor prior to operating the motor, an estimated variation outputting section for outputting an estimation variation of a temperature state by operating the motor based on the operating information, and a controlling section for controlling the temperature adjusting section to cancel a temperature change of an amount of the estimated variation in synchronism with operating the motor.