A display substrate includes an insulation substrate, an (n)-th gate line, a data line, first and second source electrodes, first and second drain electrodes, a pixel electrode, a third drain electrode, and a storage line. The first source electrode overlaps a first portion of the (n)-th gate line and is electrically connected to the data line. The first and second drain electrodes overlap a second portion of the (n)-th gate line. The pixel electrode is connected to the first drain electrode. The second source electrode overlaps a first portion of an (n+1)-th gate line, while the third drain electrode overlaps a second portion thereof. The storage line overlaps a portion of each of the pixel electrode and the second and third drain electrodes. The second source electrode is formed by extending the second drain electrode in a direction substantially from the (n)-th gate line towards the (n+1)-th gate line.