A surgical access device is provided that includes a housing having at least one access port therein for receiving a surgical instrument therethrough, and at least one elongate flexible instrument channel extending from the housing and having a lumen extending therethrough and in fluid communication with the at least one access port in the housing. The instrument channel is adapted to move from a first collapsed configuration in which the channel is sealed to substantially prevent fluid from flowing therethrough to a second expanded configuration upon insertion of an instrument therethrough.