An apparatus and method for simulating human ophthalmic artery for testing of ultrasound devices is disclosed, whereby two chambers are provided and are capable of being independently pressurized, one representing the intra-cranial space and the other representing extra-cranial space, and whereby a tube running through both chambers is provided, simulating the course of the ophthalmic artery and capable of being pressurized to stimulate arterial pressure. The apparatus is operated by pressuring both chambers, and pumping a blood-imitating fluid through the vessel in a pulsatile manner.