Invention Grant
US08206929B2 Nucleic acid amplification with allele-specific suppression of sequence variants
有权
具有等位基因特异性抑制序列变异体的核酸扩增
- Patent Title: Nucleic acid amplification with allele-specific suppression of sequence variants
- Patent Title (中): 具有等位基因特异性抑制序列变异体的核酸扩增
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Application No.: US12683111Application Date: 2010-01-06
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Publication No.: US08206929B2Publication Date: 2012-06-26
- Inventor: Michael Grow , Victoria Brophy
- Applicant: Michael Grow , Victoria Brophy
- Applicant Address: US CA Pleasanton
- Assignee: Roche Molecular Systems, Inc.
- Current Assignee: Roche Molecular Systems, Inc.
- Current Assignee Address: US CA Pleasanton
- Agent Olga Kay
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12P19/34
Abstract:
The present invention is an improved method of selective amplification of certain variants of the target sequence, enhanced by allele-specific suppression of amplification of one or more of the other variants of the target sequence. The improvement is accomplished by providing an oligonucleotide, capable of hybridizing to the desired variant of the target sequence with the lesser affinity than to the undesired variants of the target sequence and optionally, by providing chemically modified primers and hot-start conditions.
Public/Granted literature
- US20100173311A1 NUCLEIC ACID AMPLIFICATION WITH ALLELE-SPECIFIC SUPPRESSION OF SEQUENCE VARIANTS Public/Granted day:2010-07-08
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