Invention Grant
- Patent Title: Methods for diagnosis of Clostridium difficile and methods and vectors for recombinant toxin expression
- Patent Title (中): 艰难梭菌诊断方法及重组毒素表达的方法和载体
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Application No.: US12992330Application Date: 2009-05-15
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Publication No.: US08206940B2Publication Date: 2012-06-26
- Inventor: Hanping Feng , Saul Tzipori , Guilin Yang
- Applicant: Hanping Feng , Saul Tzipori , Guilin Yang
- Applicant Address: US MA Boston
- Assignee: Tufts University
- Current Assignee: Tufts University
- Current Assignee Address: US MA Boston
- Agency: Lawson & Weitzen, LLP
- Agent Sonia K. Guterman; Teofilo Javier, Jr.
- International Application: PCT/US2009/003055 WO 20090515
- International Announcement: WO2009/139919 WO 20091119
- Main IPC: G01N33/53
- IPC: G01N33/53
Abstract:
Cell-based methods for rapid real time assay of a presence of Clostridium difficile toxin and/or cells are provided, using an assay having a toxin-enhancing antibody and a sensitive cell line carrying FcyR receptors, and kits for this assay. An ultrasensitive cell based immunocytotoxicity assay for detecting less then 1 pg/ml of C. difficile toxins in clinical samples. A TcdA-specific monoclonal antibody, AIH3, was found to significantly enhance the cytotoxicity of TcdA to macrophages and monocytes. The AIH3-dependent enhancement of glucosyltransferase activity, cytoskeleton disruption, and TNF-a production induced by TcdA was demonstrated also in RAW 264.7 cells. Methods for high level recombinant expression of C. difficile toxins in Bacillus cells, and vectors for expression, strains of Bacillus carrying the vectors are provided.
Public/Granted literature
- US20110065123A1 Methods for diagnosis of Clostridium difficile and methods and vectors for recombinant toxin expression Public/Granted day:2011-03-17
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