Invention Grant
- Patent Title: Three part assay for kinase or phosphatase activity
- Patent Title (中): 三部分激酶或磷酸酶活性测定
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Application No.: US12485283Application Date: 2009-06-16
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Publication No.: US08206941B2Publication Date: 2012-06-26
- Inventor: Thilo Enderle , Doris Roth
- Applicant: Thilo Enderle , Doris Roth
- Applicant Address: US NJ Nutley
- Assignee: Hoffmann-La Roche Inc.
- Current Assignee: Hoffmann-La Roche Inc.
- Current Assignee Address: US NJ Nutley
- Agent George W. Johnston; Dennis P. Tramaloni; Brian C. Remy
- Priority: WOPCT/EP2005/053720 20050729; EP05107192 20050804
- Main IPC: C12Q1/00
- IPC: C12Q1/00 ; C12Q1/48 ; C12Q1/42 ; G01N21/00
Abstract:
The present invention relates to a method of determining kinase or phosphatase activity based on a three parts system. The method comprises contacting a binding partner which can bind phosphorylated peptides, a detection molecule and a substrate peptide. Determination of activities is achieved by measuring energy transfer between an energy donor and an energy acceptor that are present on the detection molecule and the substrate molecule.
Public/Granted literature
- US20090253150A1 Three Part Assay for Kinase or Phosphatase Activity Public/Granted day:2009-10-08
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