Invention Grant
- Patent Title: Methods for high fidelity production of long nucleic acid molecules
- Patent Title (中): 高保真生产长核酸分子的方法
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Application No.: US13019092Application Date: 2011-02-01
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Publication No.: US08206952B2Publication Date: 2012-06-26
- Inventor: Peter A. Carr , Brian Y. Chow , Joseph M. Jacobson , David W. Mosley , Christopher Emig
- Applicant: Peter A. Carr , Brian Y. Chow , Joseph M. Jacobson , David W. Mosley , Christopher Emig
- Applicant Address: US MA Cambridge
- Assignee: Massachusetts Institute of Technology
- Current Assignee: Massachusetts Institute of Technology
- Current Assignee Address: US MA Cambridge
- Agent Norma E. Henderson
- Main IPC: C12P19/34
- IPC: C12P19/34 ; C12Q1/68 ; C07H21/02 ; C07H21/04 ; C07H21/00

Abstract:
In a method for synthesizing a long nucleic acid molecule, a first immobilized nucleic acid has a first 5′ region and a first 3′ region and a second immobilized nucleic acid has a second 5′ region and a second 3′ region. The second 3′ region and the first 5′ region have identical nucleic acid sequences. An oligonucleotide is hybridized to the first 3′ region, extending the hybridized oligonucleotide and producing a first extension product having a 3′ region that is complementary to the first 5′ region. The 3′ region of the first extension product is hybridized to the second 3′ region, extending the 3′ region of the first extension product and producing a synthesized nucleic acid molecule having a 3′ region that is complementary to the second 5′ region, wherein the synthesized nucleic acid molecule has a sequence complementary to the first and second 3′ and 5′ regions.
Public/Granted literature
- US20110124055A1 Methods for High Fidelity Production of Long Nucleic Acid Molecules Public/Granted day:2011-05-26
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