Systems, methods, and computer readable media for fractional pre-emphasis of multi-mode interconnect are disclosed. According to one aspect, the subject matter described herein includes a method for fractional pre-emphasis of multi-mode interconnect. Multiple bits of binary data are periodically received. For each period, the multiple bits of binary data are encoded into multiple scalar values, each value representing a level of an analog signal to be output over a multi-mode interconnect system during the current period. Multiple analog signal outputs are generated corresponding to multiple scalar values, each signal output being driven to a level according to its corresponding scalar value. For each representative scalar value, a difference between the scalar value generated during the current period and the scalar value generated during the previous period is determined, and a pre-emphasis signal that is proportional to the difference is generated. Pre-emphasize each analog signal output according to the respective pre-emphasis signal, where the analog signal is pre-emphasized for a fraction of a period that is less than the entire period.