An electrofluidic display device including a first structure layer and a second structure layer is provided. The first structure layer includes a first substrate. A trench structure layer is disposed on the first substrate and has a trench surrounding an indent groove of a second substrate. A first electrode layer is disposed on the first substrate. A first hydrophobic layer is disposed on the first electrode layer. The second structure layer having the second substrate is located aside the first substrate with a gap. A groove structure layer is disposed on the second substrate. The groove structure has the indent groove surrounded by the trench. A second electrode layer is disposed on the groove structure layer. A second hydrophobic layer is disposed on the second electrode layer. Polar fluid is disposed in the indent groove. Non-polar fluid is disposed in the gap between the first and second substrates.