Invention Grant
US08222397B2 Methods of optimal purification of nucleic acids and kit for use in performing such methods
有权
用于实施这些方法的核酸和试剂盒的最佳纯化方法
- Patent Title: Methods of optimal purification of nucleic acids and kit for use in performing such methods
- Patent Title (中): 用于实施这些方法的核酸和试剂盒的最佳纯化方法
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Application No.: US12549685Application Date: 2009-08-28
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Publication No.: US08222397B2Publication Date: 2012-07-17
- Inventor: Rex M. Bitner
- Applicant: Rex M. Bitner
- Applicant Address: US WI Madison
- Assignee: Promega Corporation
- Current Assignee: Promega Corporation
- Current Assignee Address: US WI Madison
- Agency: Michael Best & Friedrich LLP
- Main IPC: C07H21/04
- IPC: C07H21/04
Abstract:
A method and kit which allow the use of a discrete amount of a binding matrix to first purify nucleic acids from a medium under a first set of binding conditions wherein the amount of nucleic acid bound to the binding matrix is essentially independent of the amount of surface area of the definable amount of the binding matrix, followed by a second purification step wherein the nucleic acids are bound to a discrete amount of binding matrix under a second set of binding conditions wherein the amount of nucleic acid bound to the binding matrix is essentially dependent on the amount of surface area of the definable amount of the binding matrix, thus providing a discrete quantity of nucleic acid.
Public/Granted literature
- US20110054157A1 METHODS OF OPTIMAL PURIFICATION OF NUCLEIC ACIDS AND KIT FOR USE IN PERFORMING SUCH METHODS Public/Granted day:2011-03-03
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