A DC offset cancellation circuit includes: a control signal generation unit generating i (i is a natural number) number of pulse signals having a pulse width corresponding to a DC offset amount; a current source supplying i number of currents each having a different current ratio; a switching unit determining a current quantity to be supplied to a feedback capacitor by adjusting a turn-on quantity of each of the i number of currents according to the pulse width of each of the i number of pulse signals; and an electric charge quantity regulation unit charging DC offset electric charges corresponding to current supplied from the switching unit through the feedback capacitor and transferring the DC offset electric charges charged in the feedback capacitor to a sampling capacitor through a rotary capacitor, to allow the sampling capacitor to primarily store the DC offset electric charges and then secondarily store electric charges corresponding to an input signal.