A hand-held probe for intra-operative detection of fluorescence labeled compounds includes a housing comprising a handle and a columnar portion, and a power source within the housing. A light emission source proximate the columnar portion of the housing is configured to fluoresce at least one of predetermined compounds and predetermined antibodies. An excitation switch proximate the handle selectably activates the light emission source. A detector receives fluorescent light emissions directed toward the columnar portion from at least one of the compounds and antibodies and convert the fluorescent light emissions to a corresponding emission electrical signal. A controller within the housing receives the emission electrical signal from the detector and converts the emission electrical signal to a corresponding data signal. Finally, a data port within the housing receives the data signal from the controller, converts the data signal to a corresponding output signal, and transmits the output signal.