Invention Grant
- Patent Title: Method of detecting nucleotide sequence with an intramolecular probe
- Patent Title (中): 用分子内探针检测核苷酸序列的方法
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Application No.: US12172655Application Date: 2008-07-14
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Publication No.: US08236498B2Publication Date: 2012-08-07
- Inventor: Tetsuya Tanabe , Nobuhiko Morimoto
- Applicant: Tetsuya Tanabe , Nobuhiko Morimoto
- Applicant Address: JP Tokyo
- Assignee: Olympus Corporation
- Current Assignee: Olympus Corporation
- Current Assignee Address: JP Tokyo
- Agency: Scully, Scott, Murphy & Presser, P.C.
- Priority: JP2006-013066 20060120; JP2006-353310 20061227; JP2006-353311 20061227
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12P19/34 ; C07H21/02 ; C07H21/04

Abstract:
A nucleotide sequence-detecting method, including preparing a first intramolecular detecting sequence having a sequence complementary to a first sequence located at a 3′-side of the detecting site contained in the nucleotide sample and a second intramolecular detecting sequence having a sequence complementary to a second sequence located at a 5′-side of the detecting site, preparing a detecting chain containing a sequence of the detecting chain by connecting the first intramolecular detecting sequence to the 3′ terminal of the nucleotide sample and the second intramolecular detecting sequence to the 5′ terminal, allowing intramolecular hybridization at two positions of the detecting chain, connecting the 3′ terminal of the first intramolecular detecting sequence to the 5′ terminal of the second intramolecular detecting sequence, obtaining a cyclic structure, detecting the desired sequence in the nucleotide sample from the cyclic structure.
Public/Granted literature
- US20090004666A1 METHOD OF DETECTING NUCLEOTIDE SEQUENCE WITH AN INTRAMOLECULAR PROBE Public/Granted day:2009-01-01
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