Provided is a biometric authentication method. A biometric authentication method in accordance with an aspect of the present invention includes generating a first one-time authentication template from biometric information using one-time transform information, and requesting authentication, comparing the first one-time authentication template with a one-time registration template, updating the one-time registration template and the one-time transform information when authentication is achieved according to a result of the comparison, wherein the updated one-time transform information is used to generate a second one-time authentication template to be authenticated according to the result of the comparison with the updated one-time registration template when successive authentication is requested.