Invention Grant
- Patent Title: Compositions comprising a dehalogenase substrate and a fluorescent label and methods of use
- Patent Title (中): 包含脱卤酶底物和荧光标记的组合物和使用方法
-
Application No.: US12975020Application Date: 2010-12-21
-
Publication No.: US08257939B2Publication Date: 2012-09-04
- Inventor: Keith V. Wood , Georgyi V. Los , Robert F. Bulleit , Dieter Klaubert , Mark McDougall , Chad Zimprich
- Applicant: Keith V. Wood , Georgyi V. Los , Robert F. Bulleit , Dieter Klaubert , Mark McDougall , Chad Zimprich
- Applicant Address: US WI Madison
- Assignee: Promega Corporation
- Current Assignee: Promega Corporation
- Current Assignee Address: US WI Madison
- Agency: Casimir Jones, S.C.
- Main IPC: G01N33/53
- IPC: G01N33/53 ; C12Q1/34 ; C12N9/14 ; C12P21/02 ; C07C19/00 ; C07C21/00
Abstract:
A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
Public/Granted literature
- US20110201024A1 COMPOSITIONS COMPRISING A DEHALOGENASE SUBSTRATE AND A FLUORESCENT LABEL AND METHODS OF USE Public/Granted day:2011-08-18
Information query
IPC分类:
