Invention Grant
US08257954B2 Generation of modified polymerases for improved accuracy in single molecule sequencing
有权
修饰聚合酶的生成，提高了单分子测序的准确性
- Patent Title: Generation of modified polymerases for improved accuracy in single molecule sequencing
- Patent Title (中): 修饰聚合酶的生成，提高了单分子测序的准确性
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Application No.: US12384112Application Date: 2009-03-30
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Publication No.: US08257954B2Publication Date: 2012-09-04
- Inventor: Sonya Clark , Arek Bibillo , Paul Peluso , Fred Christians , Molly He , Insil Park , Harold Lee , Keith Bjornson , Lei Jia , Robin Emig
- Applicant: Sonya Clark , Arek Bibillo , Paul Peluso , Fred Christians , Molly He , Insil Park , Harold Lee , Keith Bjornson , Lei Jia , Robin Emig
- Applicant Address: US CA Menlo Park
- Assignee: Pacific Biosciences of California, Inc.
- Current Assignee: Pacific Biosciences of California, Inc.
- Current Assignee Address: US CA Menlo Park
- Agency: Quine Intellectual Property Law Group, P.C.
- Agent Monicia Elrod-Erickson; Robert Reamey
- Main IPC: C12N9/12
- IPC: C12N9/12 ; C12P19/34
Abstract:
Provided are compositions comprising modified recombinant polymerases that exhibit branching fractions that are less than the branching fractions of the polymerases from which they were derived, or branching fractions that are less than about 25% for a phosphate-labeled nucleotide analog. Also provided are compositions comprising modified recombinant polymerases that exhibit closed polymerase/DNA complexes with increased stability relative to the parental polymerases. Also provided are compositions comprising modified recombinant polymerases that exhibit decreased rate constants relative to the parental polymerases. Provided are methods for generating polymerases with the aforementioned phenotypes. Provided are methods of using such polymerases to make a DNA or to sequence a DNA template.
Public/Granted literature
- US20100112645A1 Generation of modified polymerases for improved accuracy in single molecule sequencing Public/Granted day:2010-05-06
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