Provided are a method, system, and article of manufacture for creating playback definitions indicating segments of media content from multiple content files to render. User input is received indicating a plurality of segments of media content from a plurality of media files and an ordering of the segments. The media content comprises at least one of audio and video content. A playback definition is generated in a computer readable medium indicating the plurality of segments in the user input and the ordering of the segments. The playback definition is processed to render the media content of the indicated segments in the indicated ordering.