An LED illumination module for generating uniform stripped light source includes a stripped light-guiding unit, a stripped reflective unit and a light-emitting unit. The stripped light-guiding unit has a light-guiding body and a plurality of first light-guiding microstructures disposed on a top side of the light-guiding body. Each first light-guiding microstructure has a first reflective index. The stripped reflective unit has a reflective body corresponding to the light-guiding body and a reflective layer formed on an inner surface of the reflective body. The stripped reflective unit covers one part of the light-guiding body, the first light-guiding microstructures are disposed between the light-guiding body and the reflective layer, and the reflective layer has a second reflective index that is different from the first reflective index. The light-emitting unit has a first light-emitting module disposed beside one end of the light-guiding body.