A backlight assembly and a manufacturing method of light guide plate of the backlight assembly is provided. The light guide plate includes an incident surface to which light is incident, an exiting surface from which the light exits, and a reflection surface facing the exiting surface. The light guide plate includes recesses provided on the reflection surface recessed toward the exiting surface to scatter the light. Each of the recesses has a depth-to-width ratio of about 0.2 to about 0.33. Two prism sheets are disposed on the light guide plate. Light exits from the light guide plate at an angle of about 25 degrees to about 30 degrees.