The present invention provides a fungal bed culture of a hon-shimeji mushroom inoculated with a liquid seed culture wherein the surface of a culture medium for cultivation has a liquid seed culture inoculated portion and a liquid seed culture non-inoculated portion as well as provides a fungal bed cultivation method of a hon-shimeji mushroom which generates a fruit body from the fungal bed culture. According to the present invention, the formation rate of budlet in the fungal bed cultivation of a hon-shimeji mushroom is improved, thereby enabling stable production of a hon-shimeji mushroom in large scale commercial cultivation.