Invention Grant
- Patent Title: Sequence based engineering and optimization of single chain antibodies
- Patent Title (中): 基于序列的单链抗体工程和优化
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Application No.: US12530756Application Date: 2008-03-12
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Publication No.: US08280711B2Publication Date: 2012-10-02
- Inventor: David M. Urech , Leonardo Borras
- Applicant: David M. Urech , Leonardo Borras
- Applicant Address: CH Schlieren
- Assignee: ESBATech, an Alcon Biomedical Research Unit, LLC.
- Current Assignee: ESBATech, an Alcon Biomedical Research Unit, LLC.
- Current Assignee Address: CH Schlieren
- Agent Jason J. Derry
- International Application: PCT/EP2008/001958 WO 20080312
- International Announcement: WO2008/110348 WO 20080918
- Main IPC: G06F7/60
- IPC: G06F7/60 ; G06F19/00 ; G06F17/30
Abstract:
The invention provides methods of using sequence based analysis and rational strategies to modify and improve the structural and biophysical properties of single chain antibodies (scFvs), including stability, solubility, and antigen binding affinity. These methods and strategies can be used individually or in combination. The methods of the present invention also include the use of a database comprising scFv sequences from an experimentally screened scFv library of antibodies that have been selected to have superior solubility and stability. The invention also provides methods of using the properties found for these selected antibodies in a general approach for reshaping scFv antibodies to improve stability and solubility properties of a single chain antibody fragment.
Public/Granted literature
- US20100137150A1 Sequence Based Engineering and Optimization of Single Chain Antibodies Public/Granted day:2010-06-03
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