A display substrate includes a base substrate, a gate line, a first insulation layer, a semiconductor layer, a data line, a switching element, a light-blocking member, a reflective electrode, a second insulation layer and a transmissive electrode. The switching element is defined by a gate electrode, a source electrode and a drain electrode spaced apart from a source electrode. The light-blocking member includes a first light-blocking part disposed in correspondence with an area where the gate line and the data line are disposed, and a second light-blocking part is disposed in a reflective area to include an embossing pattern. The reflective electrode is disposed on the second light-blocking part. The second insulation layer is disposed in a pixel area of the reflective electrode. The transmissive electrode is disposed on the second insulation layer in correspondence with a transmissive area of the pixel area.