A sampling frequency offset estimation apparatus of an orthogonal frequency division multiplexing (OFDM) system includes a first differential operation unit performing complex conjugate multiplication of scattered pilots of complex symbols subjected to a fast Fourier transform (FFT) in an OFDM receiver, an interpolation unit repeating an operation of obtaining a median complex symbol between two consecutive symbols among complex symbols having first phase difference information from the first differential operation unit by a predetermined number, a second differential operation unit performing complex conjugate multiplication of two consecutive median complex symbols among median complex symbols from the interpolation unit, and a sampling frequency offset estimation unit estimating sampling frequency offset using complex symbols having second phase difference information from the second differential operation unit.